Glyphosate and glufosinate are widely used herbicides and, thus, there is an interest in the reliable and sensitive determination of glyphosate in water and food. These pesticides are difficult to extract and analyze because of their high polarity. In this report, we describe an automated workflow for the FMOC-derivatization, sample cleanup, and LC/MS/MS analysis using a GERSTEL MultiPurpose Sampler (MPS XL) confi gured with an online solid phase extraction (SPEXOS) module coupled to an AB SCIEX QTRAP® 4500 system for the identification and quantitation of glyphosate, its major metabolite AMPA, and glufosinate in water and food samples. The online SPE-LC/MS/MS method allowed detection and quantitation of all target pesticides in matrix samples at 10 μg/kg concentration levels with excellent reproducibility and values well within the ±20 % range.

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GERSTEL SPEXOS

Online SPE system with replaceable SPE cartridges: The best of all worlds

GERSTEL SPEXOS expands your sample preparation portfolio with online SPE. Compared with sandard SPE, SPEXOS relies on much smaller cartridges with only 50 mg of sorbent, enabling a significant miniaturization of the entire process: Much smaller sample amounts are needed to reach required detection limits and much less solvent is needed for analyte elution reducing cost, simplifying logistics, and increasing method sensitivity.

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