Reduction in analysis time is an important goal for easing the burden of large sample sets associated with routine screening of blood samples and sample sets produced from drug metabolism studies.

This study focuses on reduction in analysis time for simultaneous detection of delta-9-THC, 11-OH-THC, and THC-COOH in whole blood and urine extracts. This was achieved using the GERSTEL MACH fast GC system combined with an Agilent GC-MSD. Addition of the MACH system allows three independently heated temperature zones for multidimensional chromatography using Agilent Capillary Flow Technology Dean’s Switches along with fast heating/cooling rates. A novel pre-column approach protects the analytical columns in independent temperature zones and adds a high level of robustness. The Agilent Capillary Flow Technology Dean’s Switch allows a combination of heartcutting multidimensional GC and back fl ushing to reduce the amount of unwanted background components.

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Selectable 1D/2D-GC/MS

An extra GC dimension at your finger tips

Gas chromatography (GC) experts rely on sharp peaks and baseline resolution to provide accurate answers. To perform chromatographic analysis of real-world samples, analysts often must deal with either complex sample types such as essential oils and petroleum fractions, or complex matrices like biological fluids, foods, sludge, or polymers. Once the sample has been prepared for analysis, separation of all the individual compounds present by means of a single chromatographic separation can be challenging due to the compounds having different ranges of polarity, boiling point, solubility, MW, and concentration. It is therefore necessary to use innovative yet robust techniques that go beyond using a single chromatographic dimension to achieve compound separation.

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