Optimized LC separation and MS detection – the best of both worlds
In LC/MS, we work hard to reach the goal of achieving the perfect LC separation and combining it with the most efficient ionization and lowest achievable MS detection limits for our analytes.
The LC separation may require a certain pH and polarity range of the eluent, while analyte ionization in the LC/MS ionization source requires yet another pH, a different buffer – or even derivatization of the analyte for best possible efficiency or optimized spectral information. How to optimize both? The logical answer is to take the effluent from the perfect LC separation and optimize it for MS analysis.