Fat determination is one of the key analyses performed in food industry and in public food monitoring programs in order to control production processes, to ensure accurate labelling, and to monitor food safety. Therefore, a fast and reliable procedure for routine determination of fat is in great demand. Using the Cavieziel® Method, based on the fat definition given by the FDA [1], sample extraction and saponification are performed in one step, followed by gas chromatographic analysis of the resulting free fatty acids. This sample preparation method was developed to enable higher sample throughput for routine fat determination without sacrificing data quality. The total extraction time of 4 samples in parallel was reduced to 30 minutes. A predefined GC method allows the fully automated subsequent determination of total fat content and butyric acid/milk fat in one single GC run.

null
Download PDF