This application demonstrates the use of Disposable Pipette Extraction (DPX) for rapid, automated sample preparation of urine samples for comprehensive LC/MS/MS screening. The combination of automated sample cleanup and introduction with mass spectrometric detection using a Scheduled MRM™ (AB SCIEX) algorithm and fast MS/MS spectral acquisition allowed high confi dence compound identifi cation based on mass spectral library matching. The automated workflow enabled monitoring of large panels of analytes (100+ drugs); detecting and quantifying these compounds in a single run.
The new automated DPX-LC/MS/MS workflow provides rapid extractions, high recoveries, and minimized matrix interferences with complete automation capabilities towards high throughput chromatographic analysis.
