Solid Phase Micro Extraction (SPME)

Automated SPME using the GERSTEL MPS.

Solid Phase Micro Extraction (SPME) is widely used technoque for extraction and concentration of VOCs and SVOCs combined with GC/MS. SPME is especially useful in the food, flavor and beverage industries and for environmental monitoring.

Description

GERSTEL is a leading provider of SPME automation. Our systems are optimized for performance and throughput in every step of the SPME process. Up to 128 samples placed in standard Headspace vials can be analyzed in one batch.

Extraction:
  • Sample agitation with stir bars accelerates the extraction proces and improves throughput. Mechanical fiber stress is reduced, leading to longer fiber life expectancy and improved instrument up-time. The PrepAhead function allows samples to be prepared in advance while the GC run is ongoing for improved throughput and system utilization. In addition, samples can be heated or cooled during storage and extraction, which is highly useful, for example, for fresh food samples.
Desorption:
  • The Cooled Injection System (CIS) is a PTV-type GC inlet, perfectly suited for SPME analysis. The narrow-bore inlet liner ensures highly efficient analyte transfer to the GC/MS for best possible detection limits and separation efficiency. The patented Septum-Less Head (SLH) greatly reduces mechanical stress on the SPME fiber, extending its life expectancy. Septum coring and background peaks from septum material are eliminated, leading to better limits of detection and programmed heating helps ensure that analytes are introduced to the GC without thermal breakdown.
Multi-Fiber Exchange (MFX) flexibility:
  • The GERSTEL Multi Fiber Exchanger (MFX) is a unique automted fiber exchange option for the GERSTEL MPS. Using MFX, automated SPME analysis of a sample can now be performed using fibers with different polarity, covering a wide range of analytes and extracting the maximum amount of information. MFX is an excellent tool to speed up method develipment. MFX is available with a 25-fiber option of analysis of fibers that are used for remote sampling. Ready-to-use SPME Fast Fit Fiber Assemblies (FFA) for the GERSTEL MFX system can be purchased directly from Sigma-Aldrich/Supelco.
On-Fiber Derivatization:
  • Using the MPS, a derivatization reagent can be added to the fiber prior to or following the extraction step. These tasks are simply selected by mouse-click in the MAESTRO software.
Conditioning:
  • Automated conditioning in the CIS or in the MPS bake-out station ensures that the fiber is always clean and ready for the next sample. Well conditioned SPME fibers provide best possible analysis results and best limits of detection.

Documents

SPME Flyer